Complete range of screening and diagnostic solutions for Listeria

Listeria Testing Solutions

 

Monitoring and detection of food borne pathogens is a critical step in maintaining product quality and preventing product and quantify food quality indicator organisms, spoilage organisms, and bacterial foodborne pathogens. Thermo Fisher Scientific offers a broad choice of methods from traditional, selective, and differential agar media to rapid, real-time PCR assays.

 

  • Environmental Swab: SwabSURE ListeriaP - Chromogenic detection of Pathogenic Listeria from food contact and other environmental surfaces.
  • Plate Count: 3M™ Petrifilm™ facilitates easy accurate and reproducible environmental monitoring for Listeria spp.
  • Standard Culture: The ISO 11290-1:1997 method for isolation, detection and confirmation of Listeria spp. and Listeria monocytogenes contamination of food.
  • Rapid Culture: The listeria precis method is a rapid equivalent method to ISO 11290-1:1997 using selective chromogenic media following for the detection of Listeria spp. and Listeria monocytogenes in food and environmental samples.
  • Molecular Biology (qPCR): The use of qPCR for rapid detection and typing of bacterial species including Listeria spp. and Listeria monocytogenes can be performed on even the most challenging samples for food and environmental samples using our validated qPCR kits and instrumentation.

 

Comparison Chart - Which method meets my needs?

Selection Criteria

  Swab Plate Count Standard Culture Rapid Culture Molecular Biology (qPCR)
Pre-Enrichment - -

x

-

x

Selective Enrichment - -

x

- -
Plating -

x

x

x

-

Detection

x

x

x

x

x

Confirmation -

-

x

x

-
Viability -

x

x

x

-
Product QC - -

x

x

x

Environmental QC

x

x

x

x

x

Strain Identification - -

x

x

x

Quantitative -

x

x

x

-
Total Time - 27-31 hours 3-4 days 24-48 hours 8-24 hours
 

 

Environmental Swab

SwabSURE ListeriaP - Chromogenic detection of Pathogenic Listeria from food contact and other environmental surfaces

A direct test for the presumptive detection of Pathogenic Listeria monocytogenes and Listeria ivanovii from food contact and other environmental surfaces using colour change (chromogenic) technology. Includes Polystyrene shaft (breakpoint) with Pre-moistened (with Neutralising Buffer) Foam Tip Swab. Campden BRI validated.

 

  • Recommended for: food contact and environmental surfaces sampling only.
  • Ideal for: detecting pathogenic Listeria from surfaces. SwabSURE ListeriaP easily differentiates between pathogenic Listeria (L. monocytogenes & L.ivanovii) from commensal L. innocua using specific substrate technology.

 

Listeria P also inhibits other background microorganisms such as Bacillus spp., Enterococcus spp., Micrococcus spp and Gram negative organisms like Klebsiella spp and E.coli.

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Plate Count

3M™ Petriflm™ Environmental Listeria Count Plate

3M™ Petrifilm™ Environmental Listeria Plates detect the presence of Listeria in environmental samples. A sample-ready culture medium containing selective agents, nutrients, a cold water-soluble gelling agent and a chromogenic indicator. This is designed to analyse environmental samples and help increase the efficiency of monitoring plant sanitation. Rapid results in 27-31 hours from sample collection.

 

Monitor environmental Listeria levels with consistent, frequent testing to identify hot spots in your plant and track your progress. Cost effectively test more often in more locations. Zero in on the source of Listeria with a complete solution.

  • Quantitative results allow you to identify hot spots in the plant
  • Rapid results confirmed in 27–31 hours from sample collection
  • Safer method—no enrichment, no culture transfers—less risk of cross-contamination
  • No specialised equipment needed
  • Easy-to-use solution to make faster decisions, track progress against Listeria over time, and react to problem areas



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Standard Culture

Dehydrated Culture Media

Protect the quality and safety of your finished products with a vast selection of high quality base products, supplements, and special blends, including a wide range of animal-free formulations. All of our dehydrated media is tested for quality and performance before, during, and after manufacturing to make sure our finished product meets the needs of your finished product. All the familiar formulations that you use to ensure your lab performs optimally are right here at your fingertips. Dehydrated Culture Media is available under our legacy brands, Thermo Scientific Oxoid and Thermo Scientific Remel.

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Prepared Culture Media

Whether you are making 100 mL or 100 L, the same steps apply to media preparation. Eliminate many of these, from weighing out and autoclaving, to tempering, pouring or dispensing using ready-prepared media and convenience formats. Let us help you save time and labour.

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Rapid Culture

 

Chromogenic Prepared Media Plates

Colour makes a brilliant difference

 

Detect, presumptively identify, and screen samples for pathogenic and non-pathogenic organisms with speed and accuracy using chromogenic media. Colourful, easy-to-read colonies and short incubation times enable timely and important decision-making for both clinical and food testing laboratories.

 

 

Listeria plates for food and beverage

Item Code Description Pack Size Format Description for use
PP2304 Brilliance Listeria Agar 10 pcs 90 mm Plate The medium is designed to identify Listeria species based on their utilisation of a chromogenic substrate. The pathogenic Listeria spp. are then further differentiated by the detection of lecithinase (phosphotidylcholine phospholipase C-PCPLC) activity Login to buy
PP2356 Chromogenic Listeria Agar (ISO) 10 pcs 90 mm Plate The medium is designed to identify Listeria species based on their utilisation of a chromogenic substrate. The pathogenic Listeria spp. are then further differentiated by the detection of phosphotidylinositol phospholipase C (PIPLC) activity Login to buy


Learn more about our complete range of Chromogenic Prepared Media Plates

 

SureTect™ qPCR

 

SureTect™ Listeria Species PCR Assay

Detect Listeria species in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay which incorporates optimised primer and probe components for highly-specific detection of Listeria species. The internal amplification control confirms correct assay conditions have occurred and results can be relied on. The assay is AOAC and AFNOR approved.


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SureTect™ Listeria monocytogenes PCR Assay

Detect Listeria monocytogenes in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria monocytogenes PCR Assay which incorporates optimised primer and probe components for highly-specific detection of L. monocytogenes. The internal amplification control confirms correct assay conditions have occurred and results can be relied on. The assay is AOAC and AFNOR approved for additional confidence in your results.


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Validated Platforms

QuantStudio™ 5 Real-Time PCR System

The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use.

  • Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
  • User account management, locked workflow, and Pause features for greater control of experiment data
  • Built-in software features to support compliance with 21 CFR Part 11 guidelines
  • Secure cloud-based software provides increased accessibility and storage options
  • Simplified instrument software with interactive touchscreen for greater ease of use
  • 96-well 0.2mL format or 96-well 0.1mL format


Learn more

 

 

 

Complete flexibility with Applied Biosystems 7500 Fast System

Expand your in-house PCR capability beyond day-to-day pathogen testing with the Food Safety Applied Biosystems 7500 Fast Real-Time PCR instrument—a versatile, 96-well platform ideal for batch processing several hundred samples a day.



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RapidFinder™ Analysis Software v1.0

Optimise your pathogen detection workflow and enhance your downstream decision-making process with Thermo Scientific™ RapidFinder™ Analysis Software v1.0. The software simplifies the PCR run setup and the interpretation of pathogen presence/absence results.

 

RapidFinder Analysis Software and the Applied Biosystems™ QuantStudio 5 Food Safety Real-Time PCR System with a 96-well, 0.1-mL block configuration are integral parts of validated workflows for Listeria monocytogenes, Listeria spp., Salmonella spp., Salmonella species, Typhimurium and Enteritidis Multiplex, E. coli O157:H7, and Cronobacter spp. in a variety of food matrices.


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Hygiena qPCR

Hygiena BAX® System Q7

To lessen the risk of costly recalls or brand-damaging headlines, food testing labs need fast, accurate and convenient tools for detecting the presence of pathogens in their raw ingredients, environmental samples and finished products. For decades, labs around the world have chosen the award-winning BAX System as their preferred method for food safety testing.

 

With a robust list of both real-time and end-point assays globally validated for a wide variety of foods and environmental sample types, the BAX System Q7 is sure to meet the demands of food processors across the entire supply chain. The Hygiena™ BAX® System offers the accuracy and speed of advanced PCR technology in a format that is safe and simple to use and ideal for routine food testing laboratories.

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BAX® System PCR Assay Kits for Listeria

The BAX® System portfolio of Listeria assays have been designed to meet your needs,
whether you're testing food or environmental samples.

 

 

 

Item Code Description Pack Size
D11000147 BAX System Genus Listeria Assay 96 tests Login to buy
D13608135 BAX System Genus Listeria 24E Assay 96 tests Login to buy
D11000157 BAX System Listeria monocytogenes Assay 96 tests Login to buy
D13608125 BAX System Listeria monocytogenes 24E Assay 96 tests Login to buy
 

Complete flexibility with Applied Biosystems 7500 Fast System

Expand your in-house PCR capability beyond day-to-day pathogen testing with the Food Safety Applied Biosystems 7500 Fast Real-Time PCR instrument—a versatile, 96-well platform ideal for batch processing several hundred samples a day.



Request more information

 

 

Other qPCR

Applied Biosystems MicroSEQ® Pathogen Detection Kits

Easy-to-use Detection Kits
Applied Biosystems MicroSEQ® Pathogen Detection Kits offer maximum ease of use, reliability, and consistency of results. The reagents in these kits are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC) and other reagents for PCR, and no mixing is required. The kits use specially designed reaction tubes that remain closed throughout the assay process. Once the sample is added, the tubes are closed and remain that way until detection is complete, greatly reducing the chances of contamination.

 

 

 

Detection kits for Listeria spp. and Listeria monocytogenes

 

Complete flexibility with Applied Biosystems 7500 Fast System

Expand your in-house PCR capability beyond day-to-day pathogen testing with the Food Safety Applied Biosystems 7500 Fast Real-Time PCR instrument—a versatile, 96-well platform ideal for batch processing several hundred samples a day.



Request more information

 
 

Applied Biosystems QuantStudio™ 5 Real-Time PCR System

The Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use.

  • Proven OptiFlex® technology (6 decoupled channels/21 filter combinations with white LED) for trusted results
  • User account management, locked workflow, and Pause features for greater control of experiment data
  • Built-in software features to support compliance with 21 CFR Part 11 guidelines
  • Secure cloud-based software provides increased accessibility and storage options
  • Simplified instrument software with interactive touchscreen for greater ease of use
  • 96-well 0.2mL format or 96-well 0.1mL format


Learn more

 
 

RapidFinder™ Express Software v2.0

RapidFinder™ Express Software v2.0 is a graphical, easy-to-use software for multiple food pathogen detection applications. The software:

 

  • Leads you through the steps to specify the targets of interest, and the number of samples and controls to run.
  • Gives you the flexibility to let the software determine the optimal location for unknown samples and controls on the plate or to customise the plate layout.
  • Leads you through the steps to start an instrument run. The software uses preset, optimised thermal cycling conditions.
  • Automatically generates the results (presence or absence of the pathogen), which you can view for all locations, for each sample, or for individual tubes.


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